Hepatoprotective Potential of Total Anthraquinone Fraction of Rheum emodi: In vitro and In vivo Studies
Abstract
Aim: The present study aimed to evaluate the hepatoprotective potential of total Anthraquinone glycoside fraction of Rheum emodi. In vitro in freshly isolated rat hepatocytes and in vivo in wistar rats intoxicated by carbon tetrachloride (CCl4).
Methods: Dried rhizomes of rheum emodi were subjected to extraction with 70% ethanol and separation of total Anthraquinine glycoside fraction. In in-vitro study freshly isolated rat hepatocytes were treated with CCl4 and various concentrations of the total anthraquinone glycoside fractions (200-400 μg/ml). In in-vivo study, the hepatoprotective activity of total anthraquinone glycoside fraction of Rheum emodi was analyzed in liver injured CCl4 treated rats. The hepatoprotective activity was assessed using various biochemical parameters like serum bilirubin, protein, Alanine transaminase, Aspartate transaminase and alkaline phosphatase along with the Histopathological alterations.
Results: Anthraquinone glycoside fraction pre-treatment improving the removal rate of bromsulphalein from hepatic cells and also increases the cell viability this has been as a clinical evidence of hepatoprotective potential of rheum emodi. Anthraquinone glycoside administration shows significant dose dependent restoration of serum enzymes and antioxidants levels. In addition histomorphological pattern of all the experimental groups treated with Anthraquinone glycoside were significant restoration of the normal morphological pattern of hepatic cells.
Conclusion: The result obtained from the study of SOD, MDA, SGOT, SGPT and ALP clearly directing antioxidant (or) free radical scavenging property and hepatoprotective property of Rheum emodi.
