Improved Efficacy of Gliclazide with Polyherbal Formulation on Pharmacodynamic Activity in Normal and Alloxan Induced Diabetic Rats

Abstract

The present study was carried out to evaluate the hypoglycemic activity and antihyper glycemic activity of a polyherbal formulation (PHF) in normal and in alloxan induced diabetic rats. A few aqueous extracts of Vinca rosea, Gymnema sylvestre, Tinospora cordifolia, and Emblica officinalis were selected and tested for hypoglycemic and anti hyperglycemic activity. The doses which produced an optimum reduction in blood glucose levels (30-40%) were selected as ideal doses for the present study. The optimum doses of the above selected aqueous extracts of Vinca rosea, Gymnema sylvestre, Tinospora cordifolia, and Emblica officinalis were found to be 100, 100, 200 and 300 mg/kg bd.wt. So a polyherbal formulation (PHF) was prepared in the ratio of 1:1:2:3 respectively. The polyherbal formulation was evaluated at three doses 75, 150 and 300 mg/kg bd.wt. and the dose 150 mg/kg that produced an optimal reduction in blood glucose reduction was selected for the study.

Blood samples were collected at desired intervals of time and analyzed for blood glucose by GOD-POD method. Gliclazide a second generation sulfonyl urea was selected for the interaction study with that of polyherbal formulation. The doses of gliclazide (1mg/kg, 2 mg/kg and 4 mg/kg bd.wt) were given to normal and diabetic rats and the dose (2 mg/kg bd.wt) that produced an optimal reduction of blood glucose was selected for the study. The prior administration of polyherbal formulation significantly increased the hypoglycemic and antihyperglycemic activity of gliclazide.