Effect of Ezenus on Aged Rats

Abstract

Age-related changes in body are known to be related to the development of several disorders. The present study was undertaken to determine the effect of Ezenus on biochemical function and oxidative stress markers in aged rats. Animals were divided into three groups including control, moderate-dose and high-dose Ezenus treated groups. Animals were treated for a period of 90 days (twice daily) and controls were given equivalent volume of vehicle. Blood was withdrawn retroorbitally on days 0, 30, 60 and 90. Biochemical as well as oxidative stress parameters were evaluated in the sera. This included SGPT, SGOT, ALP, Glucose, Total protein, Triglycerides, HDL-C, SOD, catalase and malondialdehyde levels. Modest alteration was observed in the biochemical parameters. It was found that high dose of Ezenus prevented the minor unfavourable changes in SGPT, SGOT and ALP levels in the aged animals. Glucose, total protein, triglycerides and HDL-C levels were found to be comparable between all the groups. Interesting findings were observed in the oxidative stress markers. These parameters revealed that there was an age-dependent decline in antioxidant status of the animals with a concurrent rise in oxidative stress. Changes in oxidative stress and antioxidant levels were found to be significantly different from same groups as compared to baseline values. However, Ezenus therapy was able to significantly prevent the development of oxidative stress and maintained the levels of antioxidant enzymes as well. Ezenus therapy, at high doses, significantly prevented elevation in MDA levels. Hence, it can be concluded that Ezenus is safe at repeated dosing and also eliminates oxidative stress in rats.